Anubha Anti-Ageing Medicine

Until recently all hormone testing was performed on blood (serum). However many hormones, such as
testosterone, are bound to plasma proteins, and so measuring total hormone values, as in the case of
testosterone, is not necessarily an accurate account of free or active hormones.  Most laboratories only
evaluate serum oestradiol levels to represent the oestrogen family. However oestradiol is only a small
fraction of the bio-active oestrogens. New 24 hour urinary testing of the steroidogenic (hormone)
metabolites including progesterone and the 17 hydroxy steroids (cortisol family), the 17 keto-steroids
(DHEA and testosterone family) and the oestrogen metabolites, is providing a comprehensive review of  
hormone metabolism, which may increase diagnostic acumen.

Parent oestrogens (Oestrone and Oestradiol) are metabolised into hydroxylated forms of oestrogens
which have varying degrees of both safety and risk as far as breast, ovarian and prostate cancers are
concerned. Knowing these metabolites and their ability to be further metabolised into a water-soluble
and excretable form (methoxylated oestrogens) provides valuable information as high levels of some of
the hydroxylated oestrogens may increase the risk of oestrogen related cancers under toxic
environmental (xenoestrogenic) influences. Knowledge of the specific ratios of these metabolites directs
treatment strategies.
Urinary steroid metabolites of progesterone, Pregnanadiol and Pregnanotriol equate to used
progesterone and are a valuable diagnostic marker to assess cellular progesterone before and after
treatment. Since adequate progesterone is necessary for the conversion of inactive cortisone to active
cortisol, low progesterone levels often correlate with the clinical signs of cortisol

Cortisol metabolites can be detected through 24 hour urine testing and provide information as to the
relationship Cortisol metabolites between active cortisol and inactive cortisone through the tetrahydro
cortisol/sone metabolites. These metabolites may be relevant when symptoms of fatigue, anxiety,
depression, weight loss or gain or low libido are present.  
Testosterone and Androstenedione Metabolites assess the active metabolites of these hormones via
alpha reductase enzyme activity and inactive metabolites via beta reductase enzyme activity, suggesting
the Androgenic Index ratio.

There only a handful of laboratories worldwide which offer comprehensive steroidogenic metabolite

Careful interpretation of the results in conjuction with a patient's history and an examination is
necessary to apply the information and fine tune treatment options.

Contact Anubha to find out more about 24 hour steroidogenic testing

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